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biological experiment webpages, covering sample handling, cell or microbial culture, genetic experiments, ecological observation, and testing. Records organize source HTML, cleaned protocol text, experimental conditions, and procedural steps, and may include claims about experimental conditions, biological processes, or results paired with evidence spans, verdicts, confidence scores, and explanations. It supports training and evaluating models for evidence retrieval, claim verification, and reasoning over constraints in specialized biological web content.\u003C\u002Fp>","2026-10-15 18:04:38","2026-10-19 18:22:36","0.00",0,5000,"biological fact verification dataset,experiment protocol dataset,virtual lab data,biomedical claim verification,experimental condition extraction,evidence localization data,scientific webpage understanding,life sciences NLP","A fact verification dataset for virtual biological experiment webpages, with protocols, conditions, steps, evidence, and verdicts for specialized text and evid​","JSON","0.3GB","Biological Experiment Verification, Scientific Evidence Validation, Virtual Laboratory","Fact Verification, Evidence Retrieval, Claim Classification","V1.0","rivo\u002Fsample-packages\u002F0f634951-10e2-4c33-92ad-3c0da80b5e4c\u002F20261015.zip",[583,588,593,598,604,609,615,620,625,630,635,640],{"id":572,"name":584,"field":585,"intro":586,"type":587},"File Name","file_name","File name","string",{"id":589,"name":590,"field":591,"intro":592,"type":587},8970,"Fact Verification Verdict","verdict","The factual judgment made about the claim based on the experimental protocol text and evidence spans.",{"id":594,"name":595,"field":596,"intro":597,"type":587},8971,"Claim to Verify","claim_text","A claim about experimental conditions, biological processes, or experimental results that must be checked against the protocol text.",{"id":599,"name":600,"field":601,"intro":602,"type":603},8972,"Verification Confidence","confidence","The confidence score for the fact verification verdict, ranging from 0 to 1.","number",{"id":605,"name":606,"field":607,"intro":608,"type":587},8973,"Protocol Text","protocol_text","Protocol text extracted from the source webpage and cleaned for use.",{"id":610,"name":611,"field":612,"intro":613,"type":614},8974,"Evidence Spans","evidence_spans","Source text excerpts and their locations that support, refute, or do not directly resolve the claim.","array",{"id":616,"name":617,"field":618,"intro":619,"type":614},8975,"Protocol Steps","protocol_steps","Protocol operations organized in the order in which the experiment is performed.",{"id":621,"name":622,"field":623,"intro":624,"type":587},8976,"Protocol Category","protocol_category","The primary biological experiment category identified from the protocol content.",{"id":626,"name":627,"field":628,"intro":629,"type":587},8977,"Protocol Webpage HTML","protocol_page_html","The original webpage HTML containing sample handling, culture, genetic experiment, ecological observation, or testing protocols.",{"id":631,"name":632,"field":633,"intro":634,"type":587},8978,"Verification Rationale","verification_reason","An explanation of the verdict based on experimental conditions, biological processes, and evidence spans.",{"id":636,"name":637,"field":638,"intro":639,"type":614},8979,"Experimental Subjects","experimental_subjects","Samples, cells, microorganisms, genes, or ecological subjects extracted from the protocol.",{"id":641,"name":642,"field":643,"intro":644,"type":645},8980,"Experimental Conditions","experimental_conditions","Conditions and constraints extracted from the protocol, such as temperature, duration, culture medium, and gas environment.","object",[647,651,655,659],{"id":648,"question":649,"answer":650},6375,"What biological experiment content does the dataset cover?","It covers sample handling, cell or microbial culture, genetic experiments, ecological observation, and testing protocols. Structured information may include experimental subjects, conditions such as temperature and duration, procedural steps, and claims based on protocol content.",{"id":652,"question":653,"answer":654},6376,"Are claims paired with traceable evidence?","The schema includes evidence spans and their locations, verification verdicts, confidence scores, and explanations. These fields support analysis of whether protocol text supports or contradicts a claim. Field completeness may vary by record and should be confirmed against the delivered sample.",{"id":656,"question":657,"answer":658},6377,"Which training and evaluation tasks can this dataset support?","It can support fact verification, evidence localization, claim classification, and domain-specific text reasoning. It can also be used to evaluate whether a model can assess biological claims using experimental conditions and protocol steps.",{"id":660,"question":661,"answer":662},6378,"How can I obtain the data, and what are the licensing terms?","Check the dataset product page for available delivery options, sample specifications, and license terms. No specific license or redistribution permission is stated in this description, so confirm the applicable rights on the product page or in the contract before use.",[664,683,699],{"id":665,"relate_table":666,"relate_id":667,"file_size":572,"file_name":668,"path":8,"duration":572,"cate":483,"file_format":669,"audio_rate":8,"audio_channel":522,"audio_bit_depth":522,"quality":8,"annotation":670,"cate_name":671,"verdict":672,"claim_text":673,"confidence":674,"protocol_text":675,"evidence_spans":676,"protocol_steps":677,"protocol_category":678,"protocol_page_html":679,"verification_reason":680,"experimental_subjects":681,"experimental_conditions":682},15093,"union_cubo",136912594,"样例_3.json","json","{\"verdict\": \"Cannot determine\", \"claim_text\": \"This protocol can prove that all colonies growing on the selective medium express GFP.\", \"confidence\": \"0.94\", \"protocol_text\": \"Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\\nThen perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\\nPlate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\\nThe protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\", \"evidence_spans\": \"[{\\\"quote\\\": \\\"The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\\\", \\\"relation\\\": \\\"neutral\\\", \\\"start_offset\\\": 101, \\\"end_offset\\\": 135}]\", \"protocol_steps\": \"[\\\"Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\\\", \\\"Perform heat shock treatment and transfer into YPD recovery solution.\\\", \\\"Incubate at 30°C for 45 minutes to complete recovery.\\\", \\\"Plate the cells onto selective medium lacking the corresponding nutrient.\\\", \\\"Incubate statically at 30°C for 48 hours and screen transformants by auxotrophic complementation.\\\"]\", \"protocol_category\": \"Genetics experiment\", \"protocol_page_html\": \"\u003Carticle>\u003Ch1>Saccharomyces cerevisiae Plasmid Transformation Protocol\u003C\u002Fh1>\u003Cp>Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\u003C\u002Fp>\u003Cp>Then perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\u003C\u002Fp>\u003Cp>Plate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\u003C\u002Fp>\u003Cp>The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\u003C\u002Fp>\u003C\u002Farticle>\", \"verification_reason\": \"Growth on the selective medium only indicates that the protocol includes auxotrophic complementation screening; the text does not specify per-colony GFP fluorescence detection, nor does it provide expression results, so it cannot be used to prove that all growing colonies express GFP.\", \"experimental_subjects\": \"[\\\"Saccharomyces cerevisiae competent cells\\\", \\\"Plasmid DNA containing the GFP gene\\\", \\\"YPD recovery solution\\\", \\\"Auxotrophic selective medium\\\"]\", \"experimental_conditions\": \"{\\\"temperature\\\": 30, \\\"temperature_unit\\\": \\\"Celsius\\\", \\\"duration\\\": 48, \\\"duration_unit\\\": \\\"hours\\\", \\\"culture_medium\\\": \\\"YPD recovery solution and selective medium lacking the corresponding nutrient\\\", \\\"gas_condition\\\": \\\"not specified\\\", \\\"other_constraints\\\": [\\\"Plasmid DNA and competent cells are incubated on ice for 10 minutes\\\", \\\"After heat shock, recover in YPD recovery solution for 45 minutes\\\", \\\"Screen by auxotrophic complementation\\\", \\\"Fluorescence detection for each colony is not specified\\\"]}\"}","文本","Cannot determine","This protocol can prove that all colonies growing on the selective medium express GFP.","0.94","Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\nThen perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\nPlate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\nThe protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.","[{\"quote\": \"The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\", \"relation\": \"neutral\", \"start_offset\": 101, \"end_offset\": 135}]","[\"Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\", \"Perform heat shock treatment and transfer into YPD recovery solution.\", \"Incubate at 30°C for 45 minutes to complete recovery.\", \"Plate the cells onto selective medium lacking the corresponding nutrient.\", \"Incubate statically at 30°C for 48 hours and screen transformants by auxotrophic complementation.\"]","Genetics experiment","\u003Carticle>\u003Ch1>Saccharomyces cerevisiae Plasmid Transformation Protocol\u003C\u002Fh1>\u003Cp>Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\u003C\u002Fp>\u003Cp>Then perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\u003C\u002Fp>\u003Cp>Plate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\u003C\u002Fp>\u003Cp>The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\u003C\u002Fp>\u003C\u002Farticle>","Growth on the selective medium only indicates that the protocol includes auxotrophic complementation screening; the text does not specify per-colony GFP fluorescence detection, nor does it provide expression results, so it cannot be used to prove that all growing colonies express GFP.","[\"Saccharomyces cerevisiae competent cells\", \"Plasmid DNA containing the GFP gene\", \"YPD recovery solution\", \"Auxotrophic selective medium\"]","{\"temperature\": 30, \"temperature_unit\": \"Celsius\", \"duration\": 48, \"duration_unit\": \"hours\", \"culture_medium\": \"YPD recovery solution and selective medium lacking the corresponding nutrient\", \"gas_condition\": \"not specified\", \"other_constraints\": [\"Plasmid DNA and competent cells are incubated on ice for 10 minutes\", \"After heat shock, recover in YPD recovery solution for 45 minutes\", \"Screen by auxotrophic complementation\", \"Fluorescence detection for each colony is not specified\"]}",{"id":684,"relate_table":666,"relate_id":685,"file_size":572,"file_name":686,"path":8,"duration":572,"cate":483,"file_format":669,"audio_rate":8,"audio_channel":522,"audio_bit_depth":522,"quality":8,"annotation":687,"cate_name":671,"verdict":688,"claim_text":689,"confidence":690,"protocol_text":691,"evidence_spans":692,"protocol_steps":693,"protocol_category":694,"protocol_page_html":695,"verification_reason":696,"experimental_subjects":697,"experimental_conditions":698},15092,1682935441,"样例_2.json","{\"verdict\": \"Refuted\", \"claim_text\": \"This protocol cultures Escherichia coli K-12 for 8 hours under anaerobic conditions.\", \"confidence\": \"0.99\", \"protocol_text\": \"Seed a single colony of Escherichia coli K-12 into LB liquid medium.\\nIncubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\\nAfter cultivation, sample and measure OD600; do not interpret OD600 readings as colony-forming units.\\nThis protocol is used to obtain cultures near late log phase.\", \"evidence_spans\": \"[{\\\"quote\\\": \\\"Incubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\\\", \\\"relation\\\": \\\"refutes\\\", \\\"start_offset\\\": 25, \\\"end_offset\\\": 61}]\", \"protocol_steps\": \"[\\\"Seed a single colony of Escherichia coli K-12 into LB liquid medium.\\\", \\\"Incubate for 8 hours at 37°C with shaking at 180 rpm.\\\", \\\"Maintain aerobic aeration during cultivation.\\\", \\\"After cultivation, sample and measure OD600.\\\", \\\"Do not interpret OD600 readings as colony-forming units.\\\"]\", \"protocol_category\": \"Microbial culture\", \"protocol_page_html\": \"\u003Carticle>\u003Ch1>Escherichia coli Culture Protocol\u003C\u002Fh1>\u003Cp>Seed a single colony of Escherichia coli K-12 into LB liquid medium.\u003C\u002Fp>\u003Cp>Incubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\u003C\u002Fp>\u003Cp>After cultivation, sample and measure OD600; do not interpret OD600 readings as colony-forming units.\u003C\u002Fp>\u003Cp>This protocol is used to obtain cultures near late log phase.\u003C\u002Fp>\u003C\u002Farticle>\", \"verification_reason\": \"The protocol explicitly states that aerobic aeration is maintained during cultivation, which contradicts the anaerobic conditions claimed; although the 8-hour incubation duration matches, this cannot change the fact that the gas condition is opposite.\", \"experimental_subjects\": \"[\\\"Escherichia coli K-12\\\", \\\"LB liquid medium\\\"]\", \"experimental_conditions\": \"{\\\"temperature\\\": 37, \\\"temperature_unit\\\": \\\"Celsius\\\", \\\"duration\\\": 8, \\\"duration_unit\\\": \\\"hours\\\", \\\"culture_medium\\\": \\\"LB liquid medium\\\", \\\"gas_condition\\\": \\\"aerobic aeration\\\", \\\"other_constraints\\\": [\\\"Shaking speed is 180 rpm\\\", \\\"Measure OD600 after cultivation\\\", \\\"OD600 is not interpreted as colony-forming units\\\"]}\"}","Refuted","This protocol cultures Escherichia coli K-12 for 8 hours under anaerobic conditions.","0.99","Seed a single colony of Escherichia coli K-12 into LB liquid medium.\nIncubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\nAfter cultivation, sample and measure OD600; do not interpret OD600 readings as colony-forming units.\nThis protocol is used to obtain cultures near late log phase.","[{\"quote\": \"Incubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\", \"relation\": \"refutes\", \"start_offset\": 25, \"end_offset\": 61}]","[\"Seed a single colony of Escherichia coli K-12 into LB liquid medium.\", \"Incubate for 8 hours at 37°C with shaking at 180 rpm.\", \"Maintain aerobic aeration during cultivation.\", \"After cultivation, sample and measure OD600.\", \"Do not interpret OD600 readings as colony-forming units.\"]","Microbial culture","\u003Carticle>\u003Ch1>Escherichia coli Culture Protocol\u003C\u002Fh1>\u003Cp>Seed a single colony of Escherichia coli K-12 into LB liquid medium.\u003C\u002Fp>\u003Cp>Incubate for 8 hours at 37°C with shaking at 180 rpm, maintaining aerobic aeration during cultivation.\u003C\u002Fp>\u003Cp>After cultivation, sample and measure OD600; do not interpret OD600 readings as colony-forming units.\u003C\u002Fp>\u003Cp>This protocol is used to obtain cultures near late log phase.\u003C\u002Fp>\u003C\u002Farticle>","The protocol explicitly states that aerobic aeration is maintained during cultivation, which contradicts the anaerobic conditions claimed; although the 8-hour incubation duration matches, this cannot change the fact that the gas condition is opposite.","[\"Escherichia coli K-12\", \"LB liquid medium\"]","{\"temperature\": 37, \"temperature_unit\": \"Celsius\", \"duration\": 8, \"duration_unit\": \"hours\", \"culture_medium\": \"LB liquid medium\", \"gas_condition\": \"aerobic aeration\", \"other_constraints\": [\"Shaking speed is 180 rpm\", \"Measure OD600 after cultivation\", \"OD600 is not interpreted as colony-forming units\"]}",{"id":700,"relate_table":666,"relate_id":701,"file_size":572,"file_name":702,"path":8,"duration":572,"cate":483,"file_format":669,"audio_rate":8,"audio_channel":522,"audio_bit_depth":522,"quality":8,"annotation":703,"cate_name":671,"verdict":704,"claim_text":705,"confidence":690,"protocol_text":706,"evidence_spans":707,"protocol_steps":708,"protocol_category":709,"protocol_page_html":710,"verification_reason":711,"experimental_subjects":712,"experimental_conditions":713},15091,661543252,"样例_1.json","{\"verdict\": \"Supported\", \"claim_text\": \"This cell culture protocol requires incubation for 24 hours under 37°C and 5% CO2.\", \"confidence\": \"0.99\", \"protocol_text\": \"Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\\nIncubate in a 37°C, 5% CO2, and saturated-humidity incubator for 24 hours.\\nAfter the cells reach approximately 80% confluence, wash once with PBS, then digest with trypsin and passage.\\nThis procedure is used to maintain cell proliferation and does not include drug treatment or endpoint activity assays.\", \"evidence_spans\": \"[{\\\"quote\\\": \\\"Incubate in a 37°C, 5% CO2, and saturated-humidity incubator for 24 hours.\\\", \\\"relation\\\": \\\"supports\\\", \\\"start_offset\\\": 32, \\\"end_offset\\\": 61}]\", \"protocol_steps\": \"[\\\"Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\\\", \\\"Incubate for 24 hours under 37°C, 5% CO2, and saturated humidity.\\\", \\\"After the cells reach approximately 80% confluence, wash once with PBS.\\\", \\\"Digest the cells with trypsin and passage them.\\\"]\", \"protocol_category\": \"Cell culture\", \"protocol_page_html\": \"\u003Carticle>\u003Ch1>Cell Culture Protocol\u003C\u002Fh1>\u003Cp>Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\u003C\u002Fp>\u003Cp>Incubate in a 37°C, 5% CO2, saturated-humidity incubator for 24 hours.\u003C\u002Fp>\u003Cp>After the cells reach approximately 80% confluence, wash once with PBS, then digest with trypsin and passage.\u003C\u002Fp>\u003Cp>This procedure is used to maintain cell proliferation and does not include drug treatment or endpoint activity assays.\u003C\u002Fp>\u003C\u002Farticle>\", \"verification_reason\": \"The protocol text explicitly states 37°C, 5% CO2, and a 24-hour incubation duration, consistent with all key conditions in the claim to be verified.\", \"experimental_subjects\": \"[\\\"Adherent mammalian cells\\\", \\\"DMEM medium\\\", \\\"Trypsin\\\"]\", \"experimental_conditions\": \"{\\\"temperature\\\": 37, \\\"temperature_unit\\\": \\\"Celsius\\\", \\\"duration\\\": 24, \\\"duration_unit\\\": \\\"hours\\\", \\\"culture_medium\\\": \\\"DMEM medium containing 10% fetal bovine serum\\\", \\\"gas_condition\\\": \\\"5% CO2, saturated humidity\\\", \\\"other_constraints\\\": [\\\"Passage after the cells reach approximately 80% confluence\\\", \\\"Wash once with PBS before passage\\\", \\\"No drug treatment or endpoint activity assay\\\"]}\"}","Supported","This cell culture protocol requires incubation for 24 hours under 37°C and 5% CO2.","Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\nIncubate in a 37°C, 5% CO2, and saturated-humidity incubator for 24 hours.\nAfter the cells reach approximately 80% confluence, wash once with PBS, then digest with trypsin and passage.\nThis procedure is used to maintain cell proliferation and does not include drug treatment or endpoint activity assays.","[{\"quote\": \"Incubate in a 37°C, 5% CO2, and saturated-humidity incubator for 24 hours.\", \"relation\": \"supports\", \"start_offset\": 32, \"end_offset\": 61}]","[\"Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\", \"Incubate for 24 hours under 37°C, 5% CO2, and saturated humidity.\", \"After the cells reach approximately 80% confluence, wash once with PBS.\", \"Digest the cells with trypsin and passage them.\"]","Cell culture","\u003Carticle>\u003Ch1>Cell Culture Protocol\u003C\u002Fh1>\u003Cp>Seed adherent mammalian cells in DMEM medium containing 10% fetal bovine serum.\u003C\u002Fp>\u003Cp>Incubate in a 37°C, 5% CO2, saturated-humidity incubator for 24 hours.\u003C\u002Fp>\u003Cp>After the cells reach approximately 80% confluence, wash once with PBS, then digest with trypsin and passage.\u003C\u002Fp>\u003Cp>This procedure is used to maintain cell proliferation and does not include drug treatment or endpoint activity assays.\u003C\u002Fp>\u003C\u002Farticle>","The protocol text explicitly states 37°C, 5% CO2, and a 24-hour incubation duration, consistent with all key conditions in the claim to be verified.","[\"Adherent mammalian cells\", \"DMEM medium\", \"Trypsin\"]","{\"temperature\": 37, \"temperature_unit\": \"Celsius\", \"duration\": 24, \"duration_unit\": \"hours\", \"culture_medium\": \"DMEM medium containing 10% fetal bovine serum\", \"gas_condition\": \"5% CO2, saturated humidity\", \"other_constraints\": [\"Passage after the cells reach approximately 80% confluence\", \"Wash once with PBS before passage\", \"No drug treatment or endpoint activity assay\"]}","{\"verdict\":\"Cannot determine\",\"claim_text\":\"This protocol can prove that all colonies growing on the selective medium express GFP.\",\"confidence\":\"0.94\",\"protocol_text\":\"Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\\nThen perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\\nPlate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\\nThe protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\",\"evidence_spans\":\"[{\\\"quote\\\": \\\"The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\\\", \\\"relation\\\": \\\"neutral\\\", \\\"start_offset\\\": 101, \\\"end_offset\\\": 135}]\",\"protocol_steps\":\"[\\\"Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\\\", \\\"Perform heat shock treatment and transfer into YPD recovery solution.\\\", \\\"Incubate at 30°C for 45 minutes to complete recovery.\\\", \\\"Plate the cells onto selective medium lacking the corresponding nutrient.\\\", \\\"Incubate statically at 30°C for 48 hours and screen transformants by auxotrophic complementation.\\\"]\",\"protocol_category\":\"Genetics experiment\",\"protocol_page_html\":\"\u003Carticle>\u003Ch1>Saccharomyces cerevisiae Plasmid Transformation Protocol\u003C\\\u002Fh1>\u003Cp>Incubate Saccharomyces cerevisiae competent cells with plasmid DNA containing the GFP gene on ice for 10 minutes.\u003C\\\u002Fp>\u003Cp>Then perform heat shock treatment, transfer into YPD recovery solution, and incubate at 30°C for 45 minutes.\u003C\\\u002Fp>\u003Cp>Plate the cells onto selective medium lacking the corresponding nutrient and incubate statically at 30°C for 48 hours.\u003C\\\u002Fp>\u003Cp>The protocol only specifies screening transformants by auxotrophic complementation; it does not specify fluorescence detection for each colony.\u003C\\\u002Fp>\u003C\\\u002Farticle>\",\"verification_reason\":\"Growth on the selective medium only indicates that the protocol includes auxotrophic complementation screening; the text does not specify per-colony GFP fluorescence detection, nor does it provide expression results, so it cannot be used to prove that all growing colonies express GFP.\",\"experimental_subjects\":\"[\\\"Saccharomyces cerevisiae competent cells\\\", \\\"Plasmid DNA containing the GFP gene\\\", \\\"YPD recovery solution\\\", \\\"Auxotrophic selective medium\\\"]\",\"experimental_conditions\":\"{\\\"temperature\\\": 30, \\\"temperature_unit\\\": \\\"Celsius\\\", \\\"duration\\\": 48, \\\"duration_unit\\\": \\\"hours\\\", \\\"culture_medium\\\": \\\"YPD recovery solution and selective medium lacking the corresponding nutrient\\\", \\\"gas_condition\\\": \\\"not specified\\\", \\\"other_constraints\\\": [\\\"Plasmid DNA and competent cells are incubated on ice for 10 minutes\\\", \\\"After heat shock, recover in YPD recovery solution for 45 minutes\\\", \\\"Screen by auxotrophic complementation\\\", \\\"Fluorescence detection for each colony is not specified\\\"]}\"}","MOBIUSI 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